Bacterial Conjugation Experiment
BACTERIAL CONJUGATION EXPERIMENT
INTRODUCTION: Bacteria, in general, reproduce asexually, but in order to increase diversity, they have developed a mechanism for transfer of genetic material from one bacterium to another. The ability to perform this transfer is conferred by a set of genes which are called F for 'fertility.' These genes exist on a small, circular piece of deoxyribonucleic acid (DNA) that replicates independently from the bacterial chromosome, or they can be integrated into …
Is this Essay helpful? Join now to read this particular paper
and access over 480,000 just like this GET BETTER GRADES
and access over 480,000 just like this GET BETTER GRADES
…minutes 14 NG TNTC NG
Histadine deficient plates
Time 10-1 10-2 10-3 10-4
0 minutes 10 2 NG NG
10 minutes 46 43 26 3
20 minutes TNTC 136 NG 4
30 minutes TNTC 184 8 3
40 minutes TNTC 340 20 NG
50 minutes 45 TNTC 27 3
60 minutes 41 250 42 NG
TNTC = Too numerous to count
NG = No growth
Figure 4 - Time of entry table (percentages of recombinants)
Time Thr - Pro - His -
0 minutes .44 0 0
10 minutes 5.8 2 .02
20 minutes 6.7 2.6 .06
30 minutes .004 1.8 .08
40 minutes .0098 4.3 .09
50 minutes 1.9 2.3 .002
60 minutes 2.4 0 .11
Figure 5 - Time of entry graph (percentages of recombinants)
Figure 6 - Map of E. coli K12
Need a custom written paper? Let our professional writers save your time.
Not a member yet?
